Liquid Chromatograph Mass Spectrometer (LC-MS) is an instrument that combines liquid chromatography and mass spectrometry. It combines the efficient separation of thermally unstable and high-boiling compounds of liquid chromatography with the strong component identification capabilities of mass spectrometers. It is an effective means to separate and analyze complex organic mixtures.
Introduction of Liquid Chromatography-Mass Spectrometry
The key to online is the development of suitable interfaces. The solvent must be removed before the sample components enter the ion source. At present, crawler-type heating conveyor belts are mostly used. The downsides are:
① Components whose boiling point is similar to or lower than that of the solvent cannot be measured;
②In a sense, the advantages of HPLC separation of thermally unstable substances are lost;
③ The solvent is difficult to volatilize, and the background effect is high, which is not conducive to distinguishing.
Therefore, LC/MS is in the developmental stage and the application is not yet widespread enough.
Liquid Chromatograph Mass Spectrometer (LC-MS) is a high-end instrument in the organic analysis market. Liquid Chromatography (LC) can effectively separate the organic components in the organic samples to be tested, while Mass Spectrometry (MS) can analyze the separated organics one by one to obtain the molecular weight, structure (in some cases) and concentration ( quantitative analysis). The powerful electrospray ionization technology makes the LC-MS mass spectrum very simple, and the later data processing is simple. LC-MS is an essential analytical tool for organic matter analysis laboratories, pharmaceutical and food inspection laboratories, production process control, quality inspection and other departments.
Features of Liquid Chromatography-Mass Spectrometry
Linking liquid chromatography and mass spectrometry can add additional analytical capabilities to accurately identify and quantify trace compounds in complex sample matrices such as cell and tissue lysates, blood, plasma, urine and oral fluids. High performance liquid chromatography mass spectrometry systems (ABSciex Eksigent LC/MS and LC/MS/MS) offer some unique advantages, including:
Minimal sample preparation required for rapid analysis and transfer;
High sensitivity combined with the ability to analyze multiple compounds, even across compound classes;
High-precision, high-resolution identification and quantification of target analytes.
Application of liquid chromatography-mass spectrometry
Soil Detection Analysis Using LC/MS/MS
Detecting soil contamination, especially assessing the soil environment to which humans, animals and plants are exposed, and attempting to reduce this long-term exposure, is imperative.
Gas chromatography (GC) and liquid chromatography (LC) equipped with mass spectrometry (MS) are widely used in soil detection and analysis. Liquid chromatography in particular with triple quadrupole mass spectrometry (LC/MS/MS) offers many advantages for trace analysis of moderately polar, polar and ionic compounds in soil samples. Some
Specific applications for LC/MS/MS soil analysis include:
Identification and quantification of target multi-residue substances and general unknown substances, down to the level of ng/L;
High selectivity, ensuring the reliability of contaminant identification;
Minimal sample preparation ensures fast transfer of analytical methods and results;
Provides high sensitivity for contaminant detection, reducing the need for compound derivatization;
Soil organic matter testing provides the most relevant data results.
Currently, AxisPharm Labs is equipped with almost all types of LC/MS systems: Single-quadruple, Triple-quadruple, Ion-trap, TOF, Q-TOF, and so on. Our systems are equipped with ESI, APCI, multiple, Duo ESI, Nano sources, etc. for detecting molecules that are normally not detectable. Our LC/MS systems are also equipped with photodiode array (PDA) detector and/or evaporative light scattering detector (ELSD), reflective index detector (RID), charge aerosol detector (CAD) and fluorescence detector (FLD) for challenging detections. The combination of our LC/MS capacity enables our lab to meet wide variety of analytical needs ranging from compound characterization, quality control, and accurate mass measurement to quantitative analysis, structure elucidation, impurity profiling, and metabolite identification and so on.
LCMS Analysis
