DTSSP crosslinker reagent is a water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents. is ideal for aqueous conjugation reactions. DTSSP crosslinker can be used to crosslink extracellular and membrane proteins. If intracellular crosslinking is desired, then DSP, the water insoluble analog of DTSSP ,can be used for your research.
Properties of DTSSP
• Alternative names: Sulfo-DSP
• CAS: 81069-02-5,142702-31-6
• Molecular formula: C14H14N2Na2O14S4
• Molecular weight: 608.49
• Spacer arm length: 12.0 Å (8 atoms)
• Reactive groups: Sulfo-NHS esters, react with primary amines at pH 7.0–9.0
DTSSP is 3,3′-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.
Features and Benefits
Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
Cleavable crosslinker allows separation of crosslinked products
Water-soluble; compare with DSP
Membrane-impermeable, allowing for cell surface labeling
Applications:
• Chemical crosslinking of cell surface proteins prior to cell lysis and immunoprecipitation
• ‘Fix’ protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces
DTSSP Specifications
We manufacture DTSSP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DTSSP is tested to meet the following minimum specifications:
• Solubility: > 6 mg/mL in DI water
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of DTSSP
• Purity: > 80% by quantitative NMR (the highest standard for crosslinker purity)
Caution
This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.
